Optical Microscope is one of a kind microscope that makes use of visible light and a system of lenses to magnify images of small samples. Optical microscopes are also commonly termed as light microscope, which are the oldest known designs of microscope and were potentially invented in their current compound form in the 17th century. An optical microscope is not complex in nature; however there are many complicated designs which focus towards enhancing resolution and sample contrast. Van Leeuwenhoek's microscopes comprised of a small, solo, convex lens on a plate with an instrument to hold the material to be determined. British microscopist Brian J. Ford’s presentations have put forth some astonishingly intricate images from such basic instruments. The use of solo, convex lens to magnify objects for viewing purpose which are found today are only in the hand-lens, and the magnifying glass. The compound microscope makes use of several lenses so as to maximize the magnifying experience. The compound optical microscope has a single glass lens of short focal length for the objective, and one more solo glass lens for the ocular and eyepiece. A study done on optical microscope market categorizes it into industries, academic institutions, and several other end users including many government research institutes and private laboratories.
Mechanism of an optical microscope
All of the optical microscopes comprise of same type of basic components.
Ocular or eyepiece – a cylinder has a minimum of two or more lenses to bring the image to focus for the eye. The eyepiece is enclosed at the topmost end of the body tube. The eyepieces of the microscope are exchangeable and various types of eyepieces can be introduced along with varied magnifications. Distinctive values for eyepieces include X5, X10 and X20. The optics of the objective and eyepiece in a certain superior performing microscopes are matched to give the highest possible optical performance. Also, this is most common in apochromatic objectives.
The stage – it is a flat platform beneath the objective which backs the specimen that is being viewed. In the core of the stage is a spherical shaped hole via which the light shines to shed light on the specimen. Most of the optical microscopes have stages which have two arm-like structures to hold the slides.
The objective lens – it is a cylinder which contains one or more lenses to garner light from the sample. At the bottom end of the microscope tube one of more objective lenses are jolted into a spherical nose piece that might be rotated to pick the needed objective lens.
The illumination source – beneath the stage a light is given and controlled in many ways. In simple terms, the day light is directed with the help of a mirror. Most of the microscopes have their own light controllable source which is aimed via an optical device known as a condenser along with diaphragms and filters presented to supervise the quality and intensity of the light.